Biotinylated Human PD-1/PDCD1 Protein Recombinant

//Biotinylated Human PD-1/PDCD1 Protein Recombinant

Biotinylated Human PD-1/PDCD1 Protein Recombinant


SKU: KTKBM7KFB Category:

accession Q15116

Source DNA sequence encoding Human PD-1/PDCD1 including a C-hFc-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human PD-1/PDCD1 is a protein consisting of amino acid residues [Leu25-Gln167] ,The protein has a predicted MW of 44.7 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human PD-1/PDCD1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Immunity


Biotinylated Human PD-1/PDCD1 Protein Tris-Bis PAGE

  • Biotinylated Human PD-1 on Tris-Bis PAGE under reduced. The purity is greater than 95%.

Biotinylated Human PD-1/PDCD1 Protein SEC-HPLC

  • The purity of Biotinylated Human PD-1 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human PD-1/PDCD1 Protein ELISA Data

  • Immobilized Human PD-L1, mFc tag at 2μg/ml (100μl/Well). Dose response curve for Biotinylated Human PD-1, hFc tag with the EC50 of 0.19μg/ml determined by ELISA.