Biotinylated Human Nectin-1/PVRL1/CD111 Protein Recombinant

//Biotinylated Human Nectin-1/PVRL1/CD111 Protein Recombinant

Biotinylated Human Nectin-1/PVRL1/CD111 Protein Recombinant


SKU: KTCZZ5HHB Category:

accession Q15223

Source DNA sequence encoding Human Nectin-1/PVRL1/CD111 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human Nectin-1/PVRL1/CD111 is a protein consisting of amino acid residues [Gln31-Thr334] ,The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Nectin-1/PVRL1/CD111 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P01730 CD4_HUMAN
Interactor P15151 PVR_HUMAN
Interactor Q96NY8
Interactor Q9NQS3 NECT3_HUMAN
Biological Process Cell-adhesion
Biological Process Host-virus-interaction
Molecular function Receptor


Biotinylated Human Nectin-1/PVRL1/CD111 Protein Tris-Bis PAGE

  • Biotinylated Human Nectin-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human Nectin-1/PVRL1/CD111 Protein SEC-HPLC

  • The purity of Biotinylated Human Nectin-1 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human Nectin-1/PVRL1/CD111 Protein SPR Data

  • Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).