Biotinylated Human MSLN/Mesothelin Protein Recombinant

//Biotinylated Human MSLN/Mesothelin Protein Recombinant

Biotinylated Human MSLN/Mesothelin Protein Recombinant



accession Q13421

Source DNA sequence encoding Human MSLN/Mesothelin including a C-hFc-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human MSLN/Mesothelin is a protein consisting of amino acid residues [Glu296-Gly580] ,The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human MSLN/Mesothelin was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Cell-adhesion


Biotinylated Human MSLN/Mesothelin Protein Tris-Bis PAGE

  • Biotinylated Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human MSLN/Mesothelin Protein SEC-HPLC

  • The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC.

Biotinylated Human MSLN/Mesothelin Protein ELISA Data

  • Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA.