Biotinylated Human M-CSF R/CSF1R/CD115 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P07333
Source
DNA sequence encoding Human M-CSF R/CSF1R/CD115 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human M-CSF R/CSF1R/CD115 is a protein consisting of amino acid residues [Ile20-Glu512] ,The protein has a predicted MW of 57.4 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human M-CSF R/CSF1R/CD115 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Interactor
Biological Process
Biological Process
Molecular function
Molecular function
Methods
Biotinylated Human M-CSF R/CSF1R/CD115 Protein Tris-Bis PAGE
- Biotinylated Human M-CSF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human M-CSF R/CSF1R/CD115 Protein SEC-HPLC
- The purity of Biotinylated Human M-CSF R is greater than 95% as determined by SEC-HPLC.
Biotinylated Human M-CSF R/CSF1R/CD115 Protein ELISA Data
- Immobilized Anti-CSF R Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human M-CSF R, His Tag with the EC50 of 45.4ng/ml determined by ELISA.
Biotinylated Human M-CSF R/CSF1R/CD115 Protein SPR Data
- Biotinylated Human M-CSF R, His-Avi Tag immobilized on CM5 Chip can bind Human M-CSF, No Tag with an affinity constant of 0.81 μM as determined in SPR assay (Biacore T200).
