Biotinylated Human LAG3/CD223 Protein Recombinant

//Biotinylated Human LAG3/CD223 Protein Recombinant

Biotinylated Human LAG3/CD223 Protein Recombinant


SKU: KTF2KLOHB Category:

accession P18627

Source DNA sequence encoding Human LAG3/CD223 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human LAG3/CD223 is a protein consisting of amino acid residues [Leu23-Gly434] ,The protein has a predicted MW of 45.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human LAG3/CD223 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biotinylated Human LAG3/CD223 Protein Tris-Bis PAGE

  • Biotinylated Human LAG3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human LAG3/CD223 Protein SEC-HPLC

  • The purity of Biotinylated Human LAG3 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human LAG3/CD223 Protein ELISA Data

  • Immobilized Anti-LAG3 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human LAG3, His Tag with the EC50 of 12.7ng/ml determined by ELISA.