Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein Recombinant

//Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein Recombinant

Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein Recombinant

$618.00$2,062.00

SKU: KTG9YD7HB Category:

accession P06756


Source DNA sequence encoding Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer is a protein consisting of amino acid residues [Phe31-Val992(ITGAV) & Gly22-Asn707(ITGB6)] ,The protein has a predicted MW of 114 kDa (ITGAV)&79.1 kDa (ITGB6). Due to glycosylation, the protein migrates to 90-150 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P03231
Interactor P05556 ITB1_HUMAN
Interactor P05106
Interactor P18564

Methods

Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein Tris-Bis PAGE

  • Biotinylated Human ITGAV&ITGB6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein SEC-HPLC

  • The purity of Biotinylated Human ITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein ELISA Data

  • Immobilized Human Latent TGF beta 1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.2μg/ml determined by ELISA.