Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein Recombinant
Category: Biotinylated proteins$618.00 – $2,062.00
Description
Accession
P08648
Source
DNA sequence encoding Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer is a protein consisting of amino acid residues [Phe42-Tyr995(ITGA5)acidic tail & Gln21-Asp728(ITGB1)basic tail] ,The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
P97333
Methods
Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein Tris-Bis PAGE
- Biotinylated Human ITGA5&ITGB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein SEC-HPLC
- The purity of Biotinylated Human ITGA5&ITGB1 is greater than 90% as determined by SEC-HPLC.
Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein ELISA Data
- Immobilized 0.5μg Anti-ITGA5&ITGB1 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human ITGA5&ITGB1, His Tag with the EC50 of 55.3ng/ml determined by ELISA.
