| accession | P08648 |
| Source | DNA sequence encoding Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Molecular weight | Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer is a protein consisting of amino acid residues [Phe42-Tyr995(ITGA5)acidic tail & Gln21-Asp728(ITGB1)basic tail] ,The protein has a predicted MW of 111.8 kDa (ITGA5)&83.2 kDa (ITGB1). Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | see methods section |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | O14786 NRP1_HUMAN |
| Interactor | P05556 ITB1_HUMAN |
| Interactor | P97333 |
Methods
Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein Tris-Bis PAGE
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Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein SEC-HPLC
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Biotinylated Human Integrin alpha 5 beta 1 (ITGA5&ITGB1) Heterodimer Protein ELISA Data
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