Biotinylated Human IL-3 Protein Recombinant

//Biotinylated Human IL-3 Protein Recombinant

Biotinylated Human IL-3 Protein Recombinant



accession P08700

Source DNA sequence encoding Human IL-3 including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human IL-3 is a protein consisting of amino acid residues [Ala20-Phe152] ,The protein has a predicted MW of 16.9 kDa same as Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human IL-3 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P26951 IL3RA_HUMAN
Interactor P32927 IL3RB_HUMAN
Molecular function Cytokine
Molecular function Growth-factor


Biotinylated Human IL-3 Protein Tris-Bis PAGE

  • Biotinylated Human IL-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human IL-3 Protein SEC-HPLC

  • The purity of Biotinylated Human IL-3 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human IL-3 Protein ELISA Data

  • Immobilized Biotinylated Human IL-3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human IL-3 R alpha, hFc Tag with the EC50 of 1.3μg/ml determined by ELISA.