Biotinylated Human IL-10 Protein Recombinant

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Biotinylated Human IL-10 Protein Recombinant


SKU: KTDBU2UHB Categories: ,

accession P22301

Source DNA sequence encoding Human IL-10 including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human IL-10 is a protein consisting of amino acid residues [Ser19-Asn178] ,The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 23-25 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human IL-10 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor Q13651 I10R1_HUMAN
Interactor Q08334 I10R2_HUMAN
Molecular function Cytokine


Biotinylated Human IL-10 Protein Tris-Bis PAGE

  • Biotinylated Human IL-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human IL-10 Protein SEC-HPLC

  • The purity of Biotinylated Human IL-10 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human IL-10 Protein SPR Data

  • Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-10, His Tag with an affinity constant of 0.30 nM as determined in SPR assay (Biacore T200).