Biotinylated Human IL-1 Beta/IL-1F2 Protein Recombinant
Category: Biotinylated proteins$696.00 – $2,320.00
Description
Accession
P01584
Source
DNA sequence encoding Human IL-1 Beta/IL-1F2 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human IL-1 Beta/IL-1F2 is a protein consisting of amino acid residues [Ala117-Ser269] ,The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-1 Beta/IL-1F2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P29466
Biological Process
Molecular function
Molecular function
Molecular function
Methods
Biotinylated Human IL-1 Beta/IL-1F2 Protein SEC-HPLC
- The purity of Biotinylated Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-1 Beta/IL-1F2 Protein ELISA Data
- Immobilized Human IL-1R2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 7.6ng/ml determined by ELISA.
