Biotinylated Human IL-1 Beta/IL-1F2 Protein Recombinant

//Biotinylated Human IL-1 Beta/IL-1F2 Protein Recombinant

Biotinylated Human IL-1 Beta/IL-1F2 Protein Recombinant


SKU: KTRH804HB Category:

accession P01584

Source DNA sequence encoding Human IL-1 Beta/IL-1F2 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human IL-1 Beta/IL-1F2 is a protein consisting of amino acid residues [Ala117-Ser269] ,The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human IL-1 Beta/IL-1F2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P29466
Biological Process Inflammatory-response
Molecular function Cytokine
Molecular function Mitogen
Molecular function Pyrogen


Biotinylated Human IL-1 Beta/IL-1F2 Protein SEC-HPLC

  • The purity of Biotinylated Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.

Biotinylated Human IL-1 Beta/IL-1F2 Protein ELISA Data

  • Immobilized Human IL-1R2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 7.6ng/ml determined by ELISA.