Biotinylated Human IGF1R/CD221 Protein Recombinant

//Biotinylated Human IGF1R/CD221 Protein Recombinant

Biotinylated Human IGF1R/CD221 Protein Recombinant


SKU: KT2SMEEHB Category:

accession P08069

Source DNA sequence encoding Human IGF1R/CD221 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human IGF1R/CD221 is a protein consisting of amino acid residues [Glu31-Asn932] ,The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa (alpha subunit) and 52-55 kDa (beta subunit) based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human IGF1R/CD221 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P01730 CD4_HUMAN
Interactor P05019 IGF1_HUMAN
Interactor P27986
Interactor P29353
Interactor P35570
Interactor P81122
Interactor Q14620
Interactor Q06124
Molecular function Transferase
Molecular function Receptor


Biotinylated Human IGF1R/CD221 Protein Tris-Bis PAGE

  • Biotinylated Human IGF1R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human IGF1R/CD221 Protein SEC-HPLC

  • The purity of Biotinylated Human IGF1R is greater than 95% as determined by SEC-HPLC.

Biotinylated Human IGF1R/CD221 Protein ELISA Data

  • Immobilized Anti-IGF1R Antibody at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA.