|Source||DNA sequence encoding Human HMGB1 (Primary Amine Labeling) including a C-His tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
|Molecular weight||Recombinant Human HMGB1 (Primary Amine Labeling) is a protein consisting of amino acid residues [Met1-Glu215] ,The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 32-36 kDa based on Tris-Bis PAGE result..|
|Purity||>95%, as determined by SDS-PAGE and HPLC|
|Biological Activity||see methods section|
|Endotoxin||Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).|
|Presentation||Recombinant Human HMGB1 (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4|
|Reconstitution||Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM|
|Storage||The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.|
|Usage||This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|
Biotinylated Human HMGB1 Protein Recombinant
$542.00 – $1,805.00