| accession | P09429 |
| Source | DNA sequence encoding Human HMGB1 (Primary Amine Labeling) including a C-His tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Molecular weight | Recombinant Human HMGB1 (Primary Amine Labeling) is a protein consisting of amino acid residues [Met1-Glu215] ,The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 32-36 kDa based on Tris-Bis PAGE result.. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | see methods section |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Human HMGB1 (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P04637 P53_HUMAN |
| Biological Process | Immunity |
| Biological Process | Autophagy |
| Biological Process | Chemotaxis |
| Molecular function | DNA-binding |
Methods
Biotinylated Human HMGB1 Protein (Primary Amine Labeling) Tris-Bis PAGE
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Biotinylated Human HMGB1 Protein (Primary Amine Labeling) SEC-HPLC
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