//Biotinylated Human HMGB1 Protein Recombinant

Biotinylated Human HMGB1 Protein Recombinant

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$542.00$1,805.00

SKU: KTU2CPPHB

Description

Accession
P09429
Source
DNA sequence encoding Human HMGB1 (Primary Amine Labeling) including a C-His tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human HMGB1 (Primary Amine Labeling) is a protein consisting of amino acid residues [Met1-Glu215] ,The protein has a predicted MW of 26 kDa. Due to glycosylation, the protein migrates to 32-36 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human HMGB1 (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Biological Process
Biological Process
Biological Process
Molecular function

Methods

Biotinylated Human HMGB1 Protein (Primary Amine Labeling) Tris-Bis PAGE

  • Biotinylated Human HMGB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human HMGB1 Protein (Primary Amine Labeling) SEC-HPLC

  • The purity of Biotinylated Human HMGB1 is greater than 95% as determined by SEC-HPLC.
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