Biotinylated Human GFRAL/GFR alpha-like Protein Recombinant

//Biotinylated Human GFRAL/GFR alpha-like Protein Recombinant

Biotinylated Human GFRAL/GFR alpha-like Protein Recombinant

$542.00$1,805.00

SKU: KT08CY3HB Category:

accession Q6UXV0


Source DNA sequence encoding Human GFRAL/GFR alpha-like including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human GFRAL/GFR alpha-like is a protein consisting of amino acid residues [Ser19-Glu351] ,The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human GFRAL/GFR alpha-like was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

Biotinylated Human GFRAL/GFR alpha-like Protein Tris-Bis PAGE

  • Biotinylated Human GFRAL on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Biotinylated Human GFRAL/GFR alpha-like Protein SEC-HPLC

  • The purity of Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.

Biotinylated Human GFRAL/GFR alpha-like Protein ELISA Data

  • Immobilized Human GDF15, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 0.1μg/ml determined by ELISA.

Biotinylated Human GFRAL/GFR alpha-like Protein Blocking Data

  • Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.