Biotinylated Human GDF15 Protein Recombinant
Category: Biotinylated proteins$773.00 – $2,578.00
Description
Accession
Q99988
Source
DNA sequence encoding Human GDF15 including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human GDF15 is a protein consisting of amino acid residues [Ala197-Ile308] ,The protein has a predicted MW of 15.19 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human GDF15 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
Methods
Biotinylated Human GDF15 Protein Tris-Bis PAGE
- Biotinylated Human GDF15 on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
Biotinylated Human GDF15 Protein ELISA Data
- Immobilized Human GFRAL, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human GDF15, His Tag with the EC50 of 17.5ng/ml determined by ELISA.
