|Source||DNA sequence encoding Human GARP&Latent TGF beta 1 Complex including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
|Molecular weight||Recombinant Human GARP&Latent TGF beta 1 Complex is a protein consisting of amino acid residues [His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)] ,The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 73-78 kDa (GARP)&13 kDa&42-45 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result..|
|Purity||>95%, as determined by SDS-PAGE and HPLC|
|Biological Activity||see methods section|
|Endotoxin||Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).|
|Presentation||Recombinant Human GARP&Latent TGF beta 1 Complex was lyophilized from 0.2 µm filtered PBS solution pH7.4|
|Reconstitution||Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM|
|Storage||The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.|
|Usage||This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.|
Biotinylated Human GARP&Latent TGF beta 1 Complex Protein Recombinant
$696.00 – $2,320.00