| accession | Q14392 |
| Source | DNA sequence encoding Human GARP&Latent TGF beta 1 Complex including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Molecular weight | Recombinant Human GARP&Latent TGF beta 1 Complex is a protein consisting of amino acid residues [His20-Leu628(GARP) & Leu30-Ser390(Latent TGF beta 1)] ,The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 73-78 kDa (GARP)&13 kDa&42-45 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | see methods section |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Human GARP&Latent TGF beta 1 Complex was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P01137 TGFB1_HUMAN |
Methods
Biotinylated Human GARP&Latent TGF beta 1 Complex Protein Tris-Bis PAGE
|
Biotinylated Human GARP&Latent TGF beta 1 Complex Protein SEC-HPLC
|
Biotinylated Human GARP&Latent TGF beta 1 Complex Protein ELISA Data
|
