Biotinylated Human FGFR2 beta (IIIb) Protein Recombinant

//Biotinylated Human FGFR2 beta (IIIb) Protein Recombinant

Biotinylated Human FGFR2 beta (IIIb) Protein Recombinant

$542.00$1,805.00

SKU: KTZOTL6HB Category:

accession P21802


Source DNA sequence encoding Human FGFR2 beta (IIIb) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human FGFR2 beta (IIIb) is a protein consisting of amino acid residues [Arg152-Glu378] ,The protein has a predicted MW of 28.3 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human FGFR2 beta (IIIb) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor P09038 FGF2_HUMAN
Interactor P10767 FGF6_HUMAN
Interactor P21781 FGF7_HUMAN
Interactor O15520 FGF10_HUMAN
Interactor P01730 CD4_HUMAN
Interactor P05230 FGF1_HUMAN
Interactor P11487
Interactor P31371 FGF9_HUMAN
Interactor P50281
Molecular function Heparin-binding
Molecular function Transferase
Molecular function Receptor

Methods

Biotinylated Human FGFR2 beta (IIIb) Protein Tris-Bis PAGE

  • Biotinylated Human FGFR2 beta (IIIb) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human FGFR2 beta (IIIb) Protein SEC-HPLC

  • The purity of Biotinylated Human FGFR2 beta (IIIb) is greater than 95% as determined by SEC-HPLC.

Biotinylated Human FGFR2 beta (IIIb) Protein ELISA Data

  • Immobilized Anti-FGFR2 IIIb Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 beta (IIIb) , His Tag with the EC50 of 13.1ng/ml determined by ELISA.