Biotinylated Human FGF21 Protein Recombinant

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Biotinylated Human FGF21 Protein Recombinant


SKU: KTFL874HB Categories: ,

accession Q9NSA1

Source DNA sequence encoding Human FGF21 including a N-mFc-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human FGF21 is a protein consisting of amino acid residues [His29-Ser209] ,The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human FGF21 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Molecular function Growth-factor


Biotinylated Human FGF21 Protein Tris-Bis PAGE

  • Biotinylated Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human FGF21 Protein SEC-HPLC

  • The purity of Biotinylated Human FGF21 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human FGF21 Protein ELISA Data

  • Immobilized Biotinylated Human FGF21, mFc Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.26μg/ml determined by ELISA.