Biotinylated Human FGF21 Protein Recombinant
Categories: Biotinylated cytokinesBiotinylated proteins$542.00 – $1,805.00
Description
Accession
Q9NSA1
Source
DNA sequence encoding Human FGF21 including a N-mFc-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human FGF21 is a protein consisting of amino acid residues [His29-Ser209] ,The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human FGF21 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Biotinylated Human FGF21 Protein Tris-Bis PAGE
- Biotinylated Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human FGF21 Protein SEC-HPLC
- The purity of Biotinylated Human FGF21 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human FGF21 Protein ELISA Data
- Immobilized Biotinylated Human FGF21, mFc Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.26μg/ml determined by ELISA.
