Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P08637
Source
DNA sequence encoding Human Fc gamma RIIIA/CD16a (F176) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human Fc gamma RIIIA/CD16a (F176) is a protein consisting of amino acid residues [Gly17-Gln208(F176)] ,The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human Fc gamma RIIIA/CD16a (F176) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
Methods
Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Tris-Bis PAGE
- Biotinylated Human Fc gamma RIIIA (F176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SEC-HPLC
- The purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein ELISA Data
- Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA.
Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SPR Data
- Biotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14μM as determined in SPR assay (Biacore T200).
