Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Recombinant

//Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Recombinant

Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Recombinant

$542.00$1,805.00

SKU: KT4DPGEHB Category:

accession P08637


Source DNA sequence encoding Human Fc gamma RIIIA/CD16a (F176) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human Fc gamma RIIIA/CD16a (F176) is a protein consisting of amino acid residues [Gly17-Gln208(F176)] ,The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 48-58 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human Fc gamma RIIIA/CD16a (F176) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Molecular function IgG-binding-protein
Molecular function Receptor

Methods

Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein Tris-Bis PAGE

  • Biotinylated Human Fc gamma RIIIA (F176) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SEC-HPLC

  • The purity of Biotinylated Human Fc gamma RIIIA (F176) is greater than 95% as determined by SEC-HPLC.

Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein ELISA Data

  • Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human Fc gamma RIIIA (F176) , His Tag with the EC50 of 37.7ng/ml determined by ELISA.

Biotinylated Human Fc gamma RIIIA/CD16a (F176) Protein SPR Data

  • Biotinylated Human Fc gamma RIIIA (F176) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 0.14μM as determined in SPR assay (Biacore T200).