Biotinylated Human FAP Protein Recombinant

//Biotinylated Human FAP Protein Recombinant

Biotinylated Human FAP Protein Recombinant


SKU: KTS27I9HB Category:

accession Q12884

Source DNA sequence encoding Human FAP including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human FAP is a protein consisting of amino acid residues [Leu26-Asp760] ,The protein has a predicted MW of 87.9 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human FAP was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Angiogenesis
Biological Process Cell-adhesion
Molecular function Hydrolase


Biotinylated Human FAP Protein Tris-Bis PAGE

  • Biotinylated Human FAP on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Biotinylated Human FAP Protein SEC-HPLC

  • The purity of Biotinylated Human FAP is greater than 95% as determined by SEC-HPLC.

Biotinylated Human FAP Protein ELISA Data

  • Immobilized Anti-FAP Antibody at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FAP, His Tag with the EC50 of 0.14μg/ml determined by ELISA.