Biotinylated Human EpCAM/TROP1 Protein Recombinant

//Biotinylated Human EpCAM/TROP1 Protein Recombinant

Biotinylated Human EpCAM/TROP1 Protein Recombinant


SKU: KTFG0PSHB Category:

accession P16422

Source DNA sequence encoding Human EpCAM/TROP1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human EpCAM/TROP1 is a protein consisting of amino acid residues [Gln24-Lys245] ,The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human EpCAM/TROP1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biotinylated Human EpCAM/TROP1 Protein Tris-Bis PAGE

  • Biotinylated Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..

Biotinylated Human EpCAM/TROP1 Protein SEC-HPLC

  • The purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC.

Biotinylated Human EpCAM/TROP1 Protein ELISA Data

  • Immobilized Biotinylated Human EpCAM, His Tag at 0.2μg/ml (100ul/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.