Biotinylated Human DNAM-1/CD226 Protein Recombinant

//Biotinylated Human DNAM-1/CD226 Protein Recombinant

Biotinylated Human DNAM-1/CD226 Protein Recombinant


SKU: KTH2FLOHB Category:

accession Q15762

Source DNA sequence encoding Human DNAM-1/CD226 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human DNAM-1/CD226 is a protein consisting of amino acid residues [Glu19-Asn247] ,The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human DNAM-1/CD226 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Cell-adhesion
Molecular function Receptor


Biotinylated Human DNAM-1/CD226 Protein Tris-Bis PAGE

  • Biotinylated Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human DNAM-1/CD226 Protein SEC-HPLC

  • The purity of Biotinylated Human DNAM-1 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human DNAM-1/CD226 Protein SPR Data

  • Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human DNAM-1, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200).