Biotinylated Human DKK1 Protein Recombinant

//Biotinylated Human DKK1 Protein Recombinant

Biotinylated Human DKK1 Protein Recombinant


SKU: KTW3ITAHB Category:

accession O94907

Source DNA sequence encoding Human DKK1 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human DKK1 is a protein consisting of amino acid residues [Thr32-His266] ,The protein has a predicted MW of 28.5 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human DKK1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor O75581
Interactor O88572
Interactor Q96MU8 KREM1_HUMAN
Molecular function Developmental-protein


Biotinylated Human DKK1 Protein Tris-Bis PAGE

  • Biotinylated Human DKK1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human DKK1 Protein SEC-HPLC

  • The purity of Biotinylated Human DKK1 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human DKK1 Protein ELISA Data

  • Immobilized Biotinylated Human DKK1, His Tag at 5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human LRP-6, mFc Tag with the EC50 of 3.2ug/ml determined by ELISA.

Biotinylated Human DKK1 Protein

  • Immobilized Anti-DKK1 Antibody, hFc Tag at 0.2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human DKK1, His Tag with the EC50 of 2.3ng/ml determined by ELISA.