Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P10747
Source
DNA sequence encoding Human/Cynomolgus/Rhesus macaque CD28 including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human/Cynomolgus/Rhesus macaque CD28 is a protein consisting of amino acid residues [Asn19-Pro152] ,The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human/Cynomolgus/Rhesus macaque CD28 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P0A0L2
Interactor
P01552
Interactor
Interactor
Methods
Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein Tris-Bis PAGE
- Biotinylated Human/Cynomolgus/Rhesus macaque CD28 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein SEC-HPLC
- The purity of Biotinylated Human/Cynomolgus/Rhesus macaque CD28 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein ELISA Data
- Immobilized Anti-CD28 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA.
