| accession | P10747 |
| Source | DNA sequence encoding Human/Cynomolgus/Rhesus macaque CD28 including a N-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Molecular weight | Recombinant Human/Cynomolgus/Rhesus macaque CD28 is a protein consisting of amino acid residues [Asn19-Pro152] ,The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | see methods section |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Human/Cynomolgus/Rhesus macaque CD28 was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
| Interactor | P0A0L2 |
| Interactor | P01552 |
| Interactor | P33681 CD80_HUMAN |
| Interactor | P42081 CD86_HUMAN |
Methods
Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein Tris-Bis PAGE
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Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein SEC-HPLC
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Biotinylated Human/Cynomolgus/Rhesus macaque CD28 Protein ELISA Data
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