Biotinylated Human CTGF/CCN2 Protein Recombinant

//Biotinylated Human CTGF/CCN2 Protein Recombinant

Biotinylated Human CTGF/CCN2 Protein Recombinant



accession Q5M8T4

Source DNA sequence encoding Human CTGF/CCN2 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human CTGF/CCN2 is a protein consisting of amino acid residues [Gln27-Ala349] ,The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human CTGF/CCN2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biotinylated Human CTGF/CCN2 Protein Tris-Bis PAGE

  • Biotinylated Human CTGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human CTGF/CCN2 Protein ELISA Data

  • Immobilized Anti-CTGF Antibody at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CTGF, His Tag with the EC50 of 32.6ng/ml determined by ELISA.