Biotinylated Human CEACAM-6/CD66c Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
P40199
Source
DNA sequence encoding Human CEACAM-6/CD66c including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CEACAM-6/CD66c is a protein consisting of amino acid residues [Lys35-Gly320] ,The protein has a predicted MW of 34.1 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CEACAM-6/CD66c was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human CEACAM-6/CD66c Protein Tris-Bis PAGE
- Biotinylated Human CEACAM-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human CEACAM-6/CD66c Protein SEC-HPLC
- The purity of Biotinylated Human CEACAM-6 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human CEACAM-6/CD66c Protein SPR Data
- Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Biotinylated Human CEACAM6, His-Avi Tag with an affinity constant of 3.81 μM as determined in SPR assay (Biacore T200).
