Biotinylated Human CEACAM-6/CD66c Protein Recombinant

//Biotinylated Human CEACAM-6/CD66c Protein Recombinant

Biotinylated Human CEACAM-6/CD66c Protein Recombinant


SKU: KTI812HHB Category:

accession P40199

Source DNA sequence encoding Human CEACAM-6/CD66c including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human CEACAM-6/CD66c is a protein consisting of amino acid residues [Lys35-Gly320] ,The protein has a predicted MW of 34.1 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human CEACAM-6/CD66c was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biotinylated Human CEACAM-6/CD66c Protein Tris-Bis PAGE

  • Biotinylated Human CEACAM-6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human CEACAM-6/CD66c Protein SEC-HPLC

  • The purity of Biotinylated Human CEACAM-6 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human CEACAM-6/CD66c Protein SPR Data

  • Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Biotinylated Human CEACAM6, His-Avi Tag with an affinity constant of 3.81 μM as determined in SPR assay (Biacore T200).