Biotinylated Human CD161 Protein Recombinant

//Biotinylated Human CD161 Protein Recombinant

Biotinylated Human CD161 Protein Recombinant


SKU: KT08L9UHB Category:

accession Q12918

Source DNA sequence encoding Human CD161 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human CD161 is a protein consisting of amino acid residues [Gln67-Ser225] ,The protein has a predicted MW of 21.4 kDa. Due to glycosylation, the protein migrates to 41-45 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human CD161 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biotinylated Human CD161 Protein Tris-Bis PAGE

  • Biotinylated Human CD161 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human CD161 Protein SEC-HPLC

  • The purity of Biotinylated Human CD161 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human CD161 Protein ELISA Data

  • Immobilized Anti-CD161 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD161, His Tag with the EC50 of 41.8ng/ml determined by ELISA.