Biotinylated Human BAFF/TNFSF13B/CD257 Trimer Protein Recombinant

//Biotinylated Human BAFF/TNFSF13B/CD257 Trimer Protein Recombinant

Biotinylated Human BAFF/TNFSF13B/CD257 Trimer Protein Recombinant

$542.00$1,805.00

SKU: KTJDV2HHB Category:

accession Q9Y275


Source DNA sequence encoding Human BAFF/TNFSF13B/CD257 Trimer including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human BAFF/TNFSF13B/CD257 Trimer is a protein consisting of amino acid residues [Thr141-Leu285] ,The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human BAFF/TNFSF13B/CD257 Trimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Interactor O14836 TR13B_HUMAN
Interactor O14836 TR13B_HUMAN
Biological Process Immunity
Molecular function Cytokine

Methods

Biotinylated Human BAFF/TNFSF13B/CD257 Trimer Protein Tris-Bis PAGE

  • Biotinylated Human BAFF (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human BAFF/TNFSF13B/CD257 Trimer Protein SEC-HPLC

  • The purity of Biotinylated Human BAFF (Trimer) is greater than 95% as determined by SEC-HPLC.

Biotinylated Human BAFF/TNFSF13B/CD257 Trimer Protein ELISA Data

  • Immobilized Human BAFFR, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human BAFF (Trimer) , His Tag with the EC50 of 6.9ng/ml determined by ELISA.