Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein Recombinant

//Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein Recombinant

Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein Recombinant


SKU: KTV82KCHB Category:

accession Q5ZPR3

Source DNA sequence encoding Human B7-H3 (4Ig) /B7-H3b including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human B7-H3 (4Ig) /B7-H3b is a protein consisting of amino acid residues [Gly27-Thr461] ,The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human B7-H3 (4Ig) /B7-H3b was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein Tris-Bis PAGE

  • Biotinylated Human B7-H3 (4Ig) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein SEC-HPLC

  • The purity of Biotinylated Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.

Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein ELISA Data

  • Immobilized Anti-B7-H3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 7.7ng/ml determined by ELISA.