| accession | Q5ZPR3 |
| Source | DNA sequence encoding Human B7-H3 (4Ig) /B7-H3b including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology. |
| Molecular weight | Recombinant Human B7-H3 (4Ig) /B7-H3b is a protein consisting of amino acid residues [Gly27-Thr461] ,The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Biological Activity | see methods section |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant Human B7-H3 (4Ig) /B7-H3b was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Methods
Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein Tris-Bis PAGE
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Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein SEC-HPLC
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Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein ELISA Data
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