//Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein Recombinant

Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein Recombinant

Category:

$542.00$1,805.00

SKU: KTV82KCHB

Description

Accession
Q5ZPR3
Source
DNA sequence encoding Human B7-H3 (4Ig) /B7-H3b including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human B7-H3 (4Ig) /B7-H3b is a protein consisting of amino acid residues [Gly27-Thr461] ,The protein has a predicted MW of 49.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human B7-H3 (4Ig) /B7-H3b was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Methods

Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein Tris-Bis PAGE

  • Biotinylated Human B7-H3 (4Ig) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein SEC-HPLC

  • The purity of Biotinylated Human B7-H3 (4Ig) is greater than 95% as determined by SEC-HPLC.

Biotinylated Human B7-H3 (4Ig) /B7-H3b Protein ELISA Data

  • Immobilized Anti-B7-H3 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 of 7.7ng/ml determined by ELISA.
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