Biotinylated Human B7-H2/ICOSLG Protein Recombinant

//Biotinylated Human B7-H2/ICOSLG Protein Recombinant

Biotinylated Human B7-H2/ICOSLG Protein Recombinant


SKU: KTKVXM8HB Category:

accession O75144

Source DNA sequence encoding Human B7-H2/ICOSLG including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human B7-H2/ICOSLG is a protein consisting of amino acid residues [Asp19-Ser258] ,The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human B7-H2/ICOSLG was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Biological Process Immunity
Biological Process B-cell-activation


Biotinylated Human B7-H2/ICOSLG Protein Tris-Bis PAGE

  • Biotinylated Human B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human B7-H2/ICOSLG Protein SEC-HPLC

  • The purity of Biotinylated Human B7-H2 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human B7-H2/ICOSLG Protein ELISA Data

  • Immobilized Human ICOS (C136S,C137S) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 0.24μg/ml determined by ELISA.