Biotinylated Human B7-H2/ICOSLG Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
O75144
Source
DNA sequence encoding Human B7-H2/ICOSLG including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human B7-H2/ICOSLG is a protein consisting of amino acid residues [Asp19-Ser258] ,The protein has a predicted MW of 29.5 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human B7-H2/ICOSLG was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Methods
Biotinylated Human B7-H2/ICOSLG Protein Tris-Bis PAGE
- Biotinylated Human B7-H2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human B7-H2/ICOSLG Protein SEC-HPLC
- The purity of Biotinylated Human B7-H2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human B7-H2/ICOSLG Protein ELISA Data
- Immobilized Human ICOS (C136S,C137S) , His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC50 of 0.24μg/ml determined by ELISA.
