Biotinylated Human B7-2/CD86 Protein Recombinant

//Biotinylated Human B7-2/CD86 Protein Recombinant

Biotinylated Human B7-2/CD86 Protein Recombinant


SKU: KTZEG1WHB Category:

accession P42081

Source DNA sequence encoding Human B7-2/CD86 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then test biotinylated site specific using the AVItag biotinylation technology.test
Molecular weight Recombinant Human B7-2/CD86 is a protein consisting of amino acid residues [Leu26-Pro247] ,The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human B7-2/CD86 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P10747 CD28_HUMAN
Biological Process Immunity
Biological Process Host-virus-interaction
Molecular function Receptor


Biotinylated Human B7-2/CD86 Protein Tris-Bis PAGE

  • Biotinylated Human B7-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human B7-2/CD86 Protein ELISA Data

  • Immobilized Biotinylated Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA.