Biotinylated Human ACE2/ACEH Protein Recombinant

//Biotinylated Human ACE2/ACEH Protein Recombinant

Biotinylated Human ACE2/ACEH Protein Recombinant


SKU: KT6RF3EHB Category:

accession Q9BYF1

Source DNA sequence encoding Human ACE2/ACEH including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight Recombinant Human ACE2/ACEH is a protein consisting of amino acid residues [Gln18-Ser740] ,The protein has a predicted MW of 86.5 kDa. Due to glycosylation, the protein migrates to 95-110 kDa based on Tris-Bis PAGE result..
Purity >95%, as determined by SDS-PAGE and HPLC
Biological Activity see methods section
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant Human ACE2/ACEH was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Interactor P59594 SPIKE_CVHSA
Interactor Q6Q1S2
Biological Process Host-virus-interaction
Molecular function Hydrolase
Molecular function Receptor


Biotinylated Human ACE2/ACEH Protein Tris-Bis PAGE

  • Biotinylated Human ACE2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human ACE2/ACEH Protein SEC-HPLC

  • The purity of Biotinlylated human ACE2 is greater than 95% as determined by SEC-HPLC.

Biotinylated Human ACE2/ACEH Protein ELISA Data

  • Immobilized SARS-COV-2 S1, His Tag at 2μg/ml (100μl/Well). Dose response curve for Biotinylated Human ACE2, His Tag with EC50 of 0.24μg/ml determined by ELISA.

Biotinylated Human ACE2/ACEH Protein

  • Immobilized SARS-COV-2 Spike RBD, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ACE2, His Tag with the EC50 of 0.06μg/ml determined by ELISA.