| accession | P53 |
| Source | DNA sequence encoding the Human (HLA-A*02:01) AA25-305 [accession# P04439] and the B2M protein AA2-119 [accession# P61769], fused to the mutant P53 R175H epitope HMTEVVRHC .This complex includes a C terminal polyHis and was expressed in HEK cells,This protein was then biotinylated site specific using the AVItag biotinylation technology |
| Molecular weight | Recombinant HLA-A/P53 R175H has a calculated mass of approximately 50kDa. Due to glycosylation migrates as an approximately 60 kDa protein under reducing conditions in SDS-PAGE. |
| Purity | purity was determined to be >95% by nonreducing PAGE and HPLC (see below methods) |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant HLA-A*02:01 P53 R175H was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Methods
Biotinylated HLA-A*02:01 P53 R175H peptide complex PAGE
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Biotinylated HLA-A*02:01 P53 R175H complex HPLC
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