Biotinylated Goat Anti Human TNF-a polyclonal, antigen affinity

///Biotinylated Goat Anti Human TNF-a polyclonal, antigen affinity

Biotinylated Goat Anti Human TNF-a polyclonal, antigen affinity

$190.00$230.00


accession P01375


Applications

ELISA:This antibody can be used at 0.2 – 1.0 μg/mL jointly with Human TNF-a antibody to detect Human TNF-a. The detection limit for recombinant Human TNF-a is approximately 0.2 ng/well.

Source This antibody was produced in Rabbit immunized with recombinant Human TNF-a.
Species reactivity Human
Purification The specific antibody was purified from Rabbit sera by using immobilized recombinant Human TNF-a affinity chromatography.
Presentation Lyophilized from PBS, pH 7.2.
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


Molecular function Cytokine

Methods

Western Blot

  • The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.
  • After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20 , the membrane was incubated with biotinylated primary antibody (0.1ug/ml) at 4°C overnight.
  • Enhanced chemiluminescence (ECL) reagents were used to visualize

Sandwich ELISA

  • 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.
  • Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.
  • Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.
  • After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.
  • Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.