Biotinylated Goat Anti Human Acrp30 polyclonal, antigen affinity

///Biotinylated Goat Anti Human Acrp30 polyclonal, antigen affinity

Biotinylated Goat Anti Human Acrp30 polyclonal, antigen affinity

$190.00$230.00

SKU: RKBP062 Category: Tags: , , , ,

accession Q15848


Applications

ELISA:This antibody can be used at 0.2 – 1.0 μg/mL jointly with Human gAcrp30 antibody to detect Human gAcrp30. The detection limit for recombinant Human gAcrp30 is approximately 0.2 ng/well.

Source This antibody was produced in Rabbit immunized with recombinant Human gAcrp30.
Species reactivity Human
Purification The specific antibody was purified from Rabbit sera by using immobilized recombinant Human gAcrp30 affinity chromatography.
Presentation Lyophilized from PBS, pH 7.2.
Storage The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C. Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for 12 months without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

Western Blot

  • The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0.2 um PVDF membrane.
  • After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7.5, 500 mM NaCl, 0.1% (v/v) Tween 20 , the membrane was incubated with biotinylated primary antibody (0.1ug/ml) at 4°C overnight.
  • Enhanced chemiluminescence (ECL) reagents were used to visualize

Sandwich ELISA

  • 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed.
  • Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C.
  • Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C.
  • After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again.
  • Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.