2019 nCoV/COVID19 Spike protein S1 Recombinant-Fc

///2019 nCoV/COVID19 Spike protein S1 Recombinant-Fc

2019 nCoV/COVID19 Spike protein S1 Recombinant-Fc

$350.00$1,200.00


accession NCOVS1


Source DNA sequence encoding extracellular fragment[16-685] of NCOV Spike S1 [accession# 6VSB_A ]fused to a C terminal Human Fc IgG1 tag was expressed in HEK cells.
Molecular weight Recombinant 2019-nCoV Spike S1 is a protein consisting of 900 amino acid residues ,due to glycosylation migrates as an approximately 120 kDa protein on SDS-PAGE.
Activity The activity was tested by binding Human ACE2 in functional ELISA assay.
Purity >95%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

Recombinant SARS COV2 S1-Fc PAGE

  • 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.

SARS COV-2 Spike S1-Fc HPLC

  • The purity of 2019-nCoV Spike S1 is greater than 95% as determined by SEC-HPLC.

SARS COV2 S1 Fc ACE functional binding ELISA assay

  • 2019-ncov S1 (coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The linear range was found to be 100 ng/ml