2019 nCoV/COVID19 Spike protein S Recombinant-His
Categories: 2019-nCoV/COVID-19 CoronavirusViral Antigens$350.00 – $1,200.00
Description
Accession
NCOVS
Source
DNA sequence encoding the COVID-19 Spike S protein extracellular domain,[16-1220] accession #QIC53204.1 including a C-terminal His tag was expressed in HEK Cells.
Molecular weight
Recombinant 2019-nCoV Spike S protein is a protein consisting of the S1+S2 subunits contains 1210 amino acid residues ,due to glycosylation migrates as an approximately 120 kDa protein on SDS-PAGE.
Activity
The activity was tested by binding Human ACE2 in functional ELISA assay.
Purity
>95%, as determined by SDS-PAGE and HPLC
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant nCoV-2019 Spike S protein was lyophilized from 0.2 µm filtered 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
