2019 nCoV/COVID19 RBD Recombinant-Fc

///2019 nCoV/COVID19 RBD Recombinant-Fc

2019 nCoV/COVID19 RBD Recombinant-Fc

$350.00$1,200.00


accession NCOVRBD


Source DNA sequence encoding the COVID-19 RBD protein domain [319-541] of NCOV spike S protein[accession# 6VSB_A ]including a C-terminal mouse IgG1 tag was expressed in CHO Cells.
Molecular weight Recombinant 2019-nCoV RBD protein is a protein consisting of 460 amino acid residues ,due to glycosylation migrates as an approximately 70 kDa protein on SDS-PAGE.
Activity The activity was tested by binding Human ACE2 in functional ELISA assay.
Purity >95%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant COVID-19 RBD (Receptor Binding Domain) protein was lyophilized from 0.2 µm filtered 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

SARS COV-2 S protein RBD SDS PAGE

  • The recombinant RBD-Fc protein migrates as 70kDa due to glycosylation

SARS COV-2 RBD functional ELISA results

  • The activity was measured by the ability to bind immobilized human ACE2-Fc with a linear range of 1-50ng/ml