2019 nCoV/COVID19 Spike protein S1 Recombinant-His AVI
$350.00 – $1,200.00
| accession | NCOVS1 |
| Source | DNA sequence encoding extracellular fragment[16-675] of NCOV Spike S1 [accession# QIC53204.1] including a C terminal polyHis Avi tag was expressed in HEK cells. |
| Molecular weight | Recombinant NCOV S1 encodes the NCOV spike glycoprotein S1 sequence . This molecule has a calculated mass of approximately 76kDa. Recombinant S1 migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE. |
| Activity | The activity was tested by binding Human ACE2-Fc in functional ELISA assay, the calculated EC50 was determined to be 0.1ug/ml. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Methods
SARS COV-2 S1 SDS PAGE
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2019ncov S1-Human ACE functional binding ELISA assay
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