2019 nCoV/COVID19 Spike protein S1 Recombinant ,Biotinylated
$1,300.00 – $3,100.00
accession | NCOVS1 |
Source | DNA sequence encoding the COVID-19 Spike S1 protein extracellular domain,[16-683] (accession # QHD43416.1) fused to aq C-terminal His AVI tag was expressed in HEK Cells. |
Biotinylation | The COV2 S1 is biotinylated in vitro at a single site using the AVItagTM biotinylation technology. |
Activity | The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below). |
Molecular weight | Recombinant 2019-nCoV Spike S1 is a protein consisting of 680 amino acid residues with a calculated mW of 78kDa ,due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE. |
Purity | >95%, as determined by SDS-PAGE and HPLC |
Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
Presentation | Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |