2019 nCoV/COVID19 Spike protein S1 Recombinant ,Biotinylated

///2019 nCoV/COVID19 Spike protein S1 Recombinant ,Biotinylated

2019 nCoV/COVID19 Spike protein S1 Recombinant ,Biotinylated

$1,300.00$3,100.00


accession NCOVS1


Source DNA sequence encoding the COVID-19 Spike S1 protein extracellular domain,[16-683] (accession # QHD43416.1) fused to aq C-terminal His AVI tag was expressed in HEK Cells.
Biotinylation The COV2 S1 is biotinylated in vitro at a single site using the AVItagTM biotinylation technology.
Activity The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below).
Molecular weight Recombinant 2019-nCoV Spike S1 is a protein consisting of 680 amino acid residues with a calculated mW of 78kDa ,due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE.
Purity >95%, as determined by SDS-PAGE and HPLC
Endotoxin Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



Methods

Biotinylated SARS COV2 S1 -human ACE functional binding ELISA assay

  • Biotinylated COV2 S1 coating at 0.1ug-well) binding with Human ACE2-Fc (cat#RKQ9BYF1F) .The EC50 was determined to be 35 ng/ml

Biotinylated S1 protein PAGE

  • 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.

SARS COV-2 Spike S1 Biotinylated HPLC

  • The purity of 2019-nCoV Spike S1 is greater than 95% as determined by SEC-HPLC.