2019 nCoV/COVID19 Spike protein S1 Recombinant ,Biotinylated
$1,300.00 – $3,100.00
| accession | NCOVS1 |
| Source | DNA sequence encoding the COVID-19 Spike S1 protein extracellular domain,[16-683] (accession # QHD43416.1) fused to aq C-terminal His AVI tag was expressed in HEK Cells. |
| Biotinylation | The COV2 S1 is biotinylated in vitro at a single site using the AVItagTM biotinylation technology. |
| Activity | The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below). |
| Molecular weight | Recombinant 2019-nCoV Spike S1 is a protein consisting of 680 amino acid residues with a calculated mW of 78kDa ,due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE. |
| Purity | >95%, as determined by SDS-PAGE and HPLC |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg). |
| Presentation | Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4 |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. |
| Usage | This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes. |
Methods
Biotinylated SARS COV2 S1 -human ACE functional binding ELISA assay
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Biotinylated S1 protein PAGE
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SARS COV-2 Spike S1 Biotinylated HPLC
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