2019 nCoV/COVID19 Spike protein S1 Recombinant B.1.1.7 Variant -His
Categories: 2019-nCoV/COVID-19 CoronavirusViral Antigens$350.00 – $1,200.00
Description
Accession
NCOVS1
Source
DNA sequence encoding extracellular fragment[16-683] of NCOV Spike S1 [accession# QIC53204.1] variant B.1.1.7 (HV 69-70 deletion,Y144 deletion,N501Y,A570D,P681H)including a C terminal polyHis tag was expressed in HEK cells.
Molecular weight
Recombinant NCOV S1 B.1.1.7 encodes the NCOV spike glycoprotein S1 sequence . This molecule has a calculated mass of approximately 76kDa. Recombinant S1 migrates due to glycosylation as an approximately 120 kDa protein under reducing conditions in SDS-PAGE.
Activity
TBD
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant COVID-19 Spike S1 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
S1 variant B.1.1.7 protein PAGE
- 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
