//Biotinylated Human CD27 Ligand/CD70 Trimer Protein Recombinant

Biotinylated Human CD27 Ligand/CD70 Trimer Protein Recombinant

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$542.00$1,805.00

SKU: KTSDC4AHB

Description

Accession
P32970
Source
DNA sequence encoding Human CD27 Ligand/CD70 Trimer (Primary Amine Labeling) including a N-His tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human CD27 Ligand/CD70 Trimer (Primary Amine Labeling) is a protein consisting of amino acid residues [Leu50-Pro193] ,The protein has a predicted MW of 51.8 kDa. Due to glycosylation, the protein migrates to 62-70 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human CD27 Ligand/CD70 Trimer (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function

Methods

Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) Tris-Bis PAGE

  • Biotinylated Human CD27 Ligand (Trimer) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) SEC-HPLC

  • The purity of Biotinylated Human CD27 Ligand (Trimer) is greater than 95% as determined by SEC-HPLC.

Biotinylated Human CD27 Ligand/CD70 Trimer Protein (Primary Amine Labeling) ELISA Data

  • Immobilized Biotinylated Human CD27 Ligand (Trimer) , His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human CD27, hFc Tag with the EC50 67ng/ml determined by ELISA.
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