MERS Coronavirus

//MERS Coronavirus

Middle East respiratory syndrome-related coronavirus

MERS-CoV Genome

 

The genome of MERS-CoV is ∼30 kb and encodes 10 proteins: 2 replicase polyproteins (ORFs 1a and 1b), including the RNA-dependent RNA polymerase (RdRp); 4 structural proteins: envelope (E), M (membrane), N (nucleocapsid), and S (surface spike glycoprotein), and 4 nonstructural proteins (ORFs 3, 4a, 4b, and 5) .
There are two main recognized clades (genetic sublineages) of MERS-CoV, clade A, which includes the earliest known MERS-CoV strains (EMC/2012 and Jordan-N3/2012), and clade B, which includes newer cases (Cotten et al.
The main route of entry for MERS-CoV is dipeptidyl peptidase 4 (DPP4, also known as CD26) on the host cell surface.Within the beta-coronavirus genus, four lineages have been described: A, B (which includes SARS-CoV), C, and D. MERS-CoV is a beta-coronavirus of lineage C .
MERS-CoV can infect a variety of human cell types and tissues in culture, including macrophages, fibroblasts, and endothelial cells

The S1 subunit consists of a C domain, which contains the receptor-binding domain (RBD), and an N domain .

The S2 subunit contains a fusion peptide, the heptad repeat 1 (HR1) and HR2 domains, a transmembrane domain, and a cytoplasmic domain, which form the stalk region of S protein that facilitates fusion of the viral and cell membranes, which is necessary for virus entry .
The binding of the S1 subunit to the cellular receptor triggers conformational changes in the S2 subunit, which inserts its fusion peptide into the target cell membrane to form a six-helix bundle fusion core between the HR1 and HR2 domains that approximates the viral and cell membranes for fusion.
Neutralizing monoclonal antibodies against the RBD potently inhibit virus entry into host cells and receptor-dependent syncytium formation in cell culture, and vaccines containing the RBD induce high levels of neutralizing antibodies in mice and rabbits.

  • Source::DNA sequence encoding immunodominant fragment of SARS Spike protein was expressed in Escherichia Coli. Molecular weight:: Recombinant SARS S encodes the N terminal immunodominant sequences of the SARS Spike S1 protein. Recombinant SARS S con
  • MERS Spike protein S1 Recombinant

    $1,600.00$2,200.00
    Source::DNA sequence encoding immunodominant fragment of SARS Spike protein was expressed in Escherichia Coli. Molecular weight:: Recombinant SARS S encodes the N terminal immunodominant sequences of the SARS Spike S1 protein. Recombinant SARS S con
  • MERS Spike protein S2 Recombinant

    $1,600.00$2,200.00
    Source::DNA sequence encoding immunodominant fragment of SARS Spike protein was expressed in Escherichia Coli. Molecular weight:: Recombinant SARS S encodes the N terminal immunodominant sequences of the SARS Spike S1 protein. Recombinant SARS S con
  • Source::DNA sequence encoding immunodominant fragment of SARS Spike protein was expressed in Escherichia Coli. Molecular weight:: Recombinant SARS S encodes the N terminal immunodominant sequences of the SARS Spike S1 protein. Recombinant SARS S con