Description
Accession
Q5U334
Source
Optimized DNA sequence encoding extracellular domain of rat PVR (CD155) including a C-terminal 6His tag was expressed in HEK293 cells.
Molecular weight
Recombinant rat PVR (CD155) is a monomer protein consisting of329 amino acid residue subunits, due to glycosylation migrates as an approximately55-60 kDa protein on SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The biological activity of rat PVR was measured by the binding ability of immobilized recombinant rat CD155 (20 μg/ml ,100 μl/well ) to mouse CD226 (linear ranger of 6 - 200 ng/ml) in a functional ELISA assay.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant rat CD155is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Storage
Recombinant rat PVR (CD155), as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only. It may not be used for therapeutics or diagnostic purposes.
