/, Biotinylated proteins/Biotinylated Human IL-18 Protein Recombinant

Biotinylated Human IL-18 Protein Recombinant

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$542.00$1,805.00

SKU: KT2GVC5HB

Description

Accession
Q14116
Source
DNA sequence encoding Human IL-18 (Primary Amine Labeling) including a No Tag tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human IL-18 (Primary Amine Labeling) is a protein consisting of amino acid residues [Tyr37-Asp193] ,The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-18 (Primary Amine Labeling) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Q9IW12
Interactor
Q99665
Molecular function

Methods

Biotinylated Human IL-18 Protein (Primary Amine Labeling) Tris-Bis PAGE

  • Biotinylated Human IL-18 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated Human IL-18 Protein (Primary Amine Labeling) ELISA Data

  • Immobilized Human IL-18BP, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 26.0ng/ml determined by ELISA.
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