Biotinylated Human IL-13 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
AAK53823
Source
DNA sequence encoding Human IL-13 including a N-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human IL-13 is a protein consisting of amino acid residues [Gly21-Asn132] ,The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human IL-13 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human IL-13 Protein Tris-Bis PAGE
- Biotinylated IL-13 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Biotinylated Human IL-13 Protein ELISA Data
- Immobilized Biotinylated Human IL-13, His Tag at 2μg/ml (100μl/well) on the streptavidin (5μg/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 1.4μg/ml determined by ELISA.
