bovine Basic Fibroblast Growth Factor Recombinant
Categories: Bovine growth factorsFood grade growth factorsFGF family$120.00 – $500.00
Description
Accession
P03969
Source
Optimized DNA sequence encoding Bovine Basic Fibroblast Growth Factor mature chain sp|P03969|10-155 was expressed in Escherichia Coli.
Molecular weight
Recombinant bovine basic FGF is a monomer protein, consisting of 145 amino acids and migrates as an approximately 17kDa protein under reducing conditions.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the proliferation of NR6R-3T3 fibroblasts, was found to be less than 0.1 ng/ml,corresponding tox106 IU/mg.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant bovine bFGF was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Biological Process
Molecular function
Molecular function
Molecular function