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Mouse Hepatocyte Growth Factor Recombinant

HGF

accession:  Q08048
   Size A     20ug   $ 160
   Size B      100ug  $680
   Size C      500ug  $ 2700
Domain  :  Kringles,PAN,peptidase S1
Gene  :  HGF
Catalog no. :  RKQ08048
Source:
Optimized DNA sequence encoding Mouse Hepatocyte Growth Factor mature chain was expressed in Chinese Hamster Ovary cell line.

 
Molecular weight:
Native human Hepatocyte Growth Factor is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 80 kDa.

Recombinant HGF  is a glycosylated disulfide-linked homodimeric protein consisting of alpha chain (463) and the beta chain (233) amino acid residue subunits, and migrates as an approximately 75 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of murine  BNL-CL2 cell li was found to be in the range of 20.0-40.0 ng/ml.

 
Protein Sequence:
MMWGTKLLPV LLLQHVLLHL LLLHVAIPYA EGQKKRRNTL HEFKKSAKTT    50
LTKEDPLLKI KTKKVNSADE CANRCIRNRG FTFTCKAFVF DKSRKRCYWY 100
PFNSMSSGVK KGFGHEFDLY ENKDYIRNCI IGKGGSYKGT VSITKSGIKC 150
QPWNSMIPHE HSFLPSSYRG KDLQENYCRN PRGEEGGPWC FTSNPEVRYE 200
VCDIPQCSEV ECMTCNGESY RGPMDHTESG KTCQRWDQQT PHRHKFLPER 250
YPDKGFDDNY CRNPDGKPRP WCYTLDPDTP WEYCAIKTCA HSAVNETDVP 300
METTECIQGQ GEGYRGTSNT IWNGIPCQRW DSQYPHKHDI TPENFKCKDL 350
RENYCRNPDG AESPWCFTTD PNIRVGYCSQ IPKCDVSSGQ DCYRGNGKNY 400
MGNLSKTRSG LTCSMWDKNM EDLHRHIFWE PDASKLNKNY CRNPDDDAHG 450
PWCYTGNPLI PWDYCPISRC EGDTTPTIVN LDHPVISCAK TKQLR>VVNGI 500
PTQTTVGWMV SLKYRNKHIC GGSLIKESWV LTARQCFPAR NKDLKDYEAW 550
LGIHDVHERG EEKRKQILNI SQLVYGPEGS DLVLLKLARP AILDNFVSTI 600
DLPSYGCTIP EKTTCSIYGW GYTGLINADG LLRVAHLYIM GNEKCSQHHQ 650
GKVTLNESEL CAGAEKIGSG PCEGDYGGPL ICEQHKMRMV LGVIVPGRGC 700
AIPNRPGIFV RVAYYAKWIH KVILTYKL
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Mouse HGF was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

HGF

Hepatocyte growth factor (HGF) is a multifunctional cytokine that was originally described as a mesenchymal- derived factor that regulates cell growth, cell motility, morphogenesis and angiogenesis through activation of its receptor, the transmembrane tyrosine kinase encoded by the c-Met proto-oncogene. HGF and c- Met are often co-expressed or over-expressed in a variety of human malignancies including glioblastoma and medullablastoma. The multifunctional effects of HGF:c-Met signaling in tumor cells are mediated by a network of signal transduction pathways including mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K). HGF consists of two subunits held by a disulfide bond. The alpha subunit (69 kDa) has a length of 440 amino acids. The beta subunit (34 kDa) has a length of 234 amino acids. The beta chain of the factor shows approximately 38 % homology at the protein level to the serine protease domain of plasminogen. A variant of the beta chain has been shown to function as a subunit of a hybrid cytokine known as PPBSF [pre-pro-B- cell growth stimulating factor]. HGF is a glycoprotein; the non-glycosylated factor is also bioactive. The beta- chain of the murine and rat factor display approximately 95 % sequence identity. The murine beta-chain also shows approximately 70 % sequence identity with rabbit HPTA.

Related Publications:
HGF family negatively regulates hepatic gluconeogenesis via induction of orphan nuclear receptor shp in primary hepatocytes
J. Biol. Chem., Aug 2009; 10.1074/jbc.M109.022244.
HGF/met signalling promotes pge2 biogenesis via regulation of cox-2 and 15-pgdh expression in colorectal cancer cells
Carcinogenesis, Aug 2009; 10.1093/carcin/bgp183.
the angiogenic growth factors HGF and vegf in serum and plasma from neuroblastoma patients
Anticancer Res, Aug 2009; 29: 3311 - 3319.
possible implication of satellite cells in regenerative motoneuritogenesis: HGF upregulates neural chemorepellent sema3a during myogenic differentiation
Am J Physiol Cell Physiol, Aug 2009; 297: C238 - C252.
HGF and c-met participate in paracrine tumorigenic pathways in head and neck squamous cell cancer
Clin. Cancer Res., Jun 2009; 15: 3740 - 3750.




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