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Human Adiponectin Recombinant (CHO Cell)

Adiponectin

accession:  P21583
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      1mg  $ 3500
Domain  :  four helix cytokine core
Gene  :  PIGF
Catalog no. :  RCQ15848
Source:
Optimized DNA sequence encoding Human Adiponectin (ACRP30) mature chain fused to a C terminal His tag was expressed in CHO Cells

 
Molecular weight:

Native human Adiponectin is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 25kDa.

Recombinant Adiponectin(ACRP30) is a  protein consisting of 234 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the cytotoxic assy of  human M1 cells , and determined to be  ≤ 5ug/ml.

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Adiponectin  was lyophilized from a 0.2 μm filtered solution inPBS pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

Adiponectin

Adiponectin is an adipocyte- produced cytokine (adipokine) that circulates at high concentrations.It regulates inflammation (1) and is known for regulating glucose and lipid metabolism through its insulin- sensitizing effects. The adipokine is secreted as a monomeric protein that oligomerizes to form a low- molecular-weight (LMW) trimer, a middle-molecular- weight (MMW) hexamer, and a high-molecular-weight (HMW) 12- to 18-mer. Low adiponectin concentrations mediate obesity-linked diseases such as type 2 diabetes and cardiovascular diseases.

Related Publications:
baseline Adiponectin levels do not influence the response to pioglitazone in act now
Diabetes Care, Apr 2014; 10.2337/dc13-1745.
no association of plasma levels of Adiponectin and c-peptide with risk of aggressive prostate cancer in the cancer prevention study ii nutrition cohort
Cancer Epidemiol. Biomarkers Prev., Apr 2014; 10.1158/1055-9965.EPI-14-0114.
are baseline high molecular weight Adiponectin levels associated with radiographic progression in rheumatoid arthritis and osteoarthritis?
J Rheumatol, Apr 2014; 10.3899/jrheum.130888.
Adiponectin is sufficient, but not required, for exercise-induced increases in the expression of skeletal muscle mitochondrial enzymes.
J. Physiol., Mar 2014; 10.1113/jphysiol.2014.273680.




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Antibodies:
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product_icon_8.jpg Human Adiponectin Recombinant (CHO Cell)
 
 
 
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