/, Cytokines/Human Oncostatin M Recombinant (CHO Cell)

Human Oncostatin M Recombinant (CHO Cell)

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$160.00$4,700.00

SKU: RCP13725 Tags: , ,

Description

Accession
P13725
Source
Optimized DNA sequence encoding Human Oncostatin-M mature chain was expressed in CHO Cells
Molecular weight
Native human Oncostatin-M is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 25 kDa. Recombinant Oncostatin-M is a protein consisting of 227 amino acid residue subunits, due to glycosylation migrates as an approximately 25 kDa protein under reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependent stimulation of the proliferation off human TF-1 cells is ≤3 ng/ml, corresponding to a specific activity of ≥2 x units/mg.
Protein Sequence
MGVLLTQRTL LSLVLALLFP SMASMAAIGS CSKEYRVLLG QLQKQTDLMQ DTSRLLDPYI RIQGLDVPKL REHCRERPGA FPSEETLRGL GRRGFLQTLN ATLGCVLHRL ADLEQRLPKA QDLERSGLNI EDLEKLQMAR PNILGLRNNI YCMAQLLDNS DTAEPTKAGR GASQPPTPTP ASDAFQRKLE GCRFLHGYHR FMHSVGRVFS KWGESPNRSR RHSPHQALRK GVRRTRPSRK GKRLMTRGQL PR
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant Oncostatin M was lyophilized from a.2μm filtered concentrated (1mg/ml) PBS solution, pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Biological Process
Molecular function
Molecular function
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