Optimized DNA sequence encodingÂ Human Carnosinase -CNDP1 (SER28 - HIS508) mature chain including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Carnosinase (CN1) is a protein consisting of 488 amino acid residue subunits,due to glycosylation migrates as an approximately as 69kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was measured by the ability to cleave carnosine, the activity was calculated to be >250 pmol/min/µg
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/Âµg(1EU/Âµg).
Recombinant human Carnosinase (CNDP1) is lyophilized Â from 0.2 Î¼m filtered PBS solution, Â pH7.2 , Â 5% Trehalose.
Â Â Â Â Â Â Â Â
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Human Carnosinase (CNDP1)can be stored in working aliquots at 2Â° - 8Â° C for one month, or at -20Â°C toÂ -70Â°CÂ for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Serum carnosinase, a
dipeptidase belonging to the
M20 metalloprotease family,
is the rate-limiting enzyme
for the hydrolysis of
carnosine into β-alanine and
histidine. Carnosine is an
nonenzymatic glycosylation ,
prevents high glucose–
induced extracellular matrix
accumulation, and prevents
cross-linking of proteins
caused by reactive aldehydes
does low serum Carnosinase activity favor high-intensity exercise capacity?
J Appl Physiol,
553 - 559.
low plasma Carnosinase activity promotes carnosinemia after carnosine ingestion in humans
Am J Physiol Renal Physiol,
F1537 - F1544.
n-glycosylation of Carnosinase influences protein secretion and enzyme activity: implications for hyperglycemia
1984 - 1990.
structural basis for substrate recognition and hydrolysis by mouse Carnosinase cn2
J. Biol. Chem.,
27289 - 27299.
exclusion of polymorphisms in Carnosinase genes (cndp1 and cndp2) as a cause of diabetic nephropathy in type 1 diabetes: results of large case-control and follow-up studies
2547 - 2551.